Routine PCR and real-time PCR

Molecular diagnostic reagent and analyzer.
High sensitivity, high specificity, easy to use, fast analytical timing and limited costs. Wide range of technical tests based on Real-Time Polymerase Chain Reaction (PCR) technique (PCR RFLP), Sanger sequencing, PCR Cold (RFLP) Cold PCR Sanger sequencing. Wide range of technical tests based on Real-Time Polymerase Chain Reaction (PCR) technique such as PCR RFLP, COLD PCR and Sanger sequencing. These products are validated on the most common platforms as Rotor-Gene™ (Qiagen), LineGeneKTM (Bioer Technologies), iQ5, CFXTM (BioRad), SmartCycler™ (Cepheid), Applied Biosystems 7300/7500, StepOne, MX3005PTM, MX3000 PTM (Agilent Technologies)

SARS-CoV-2 Triplex PCR kit

REF 89-03 Form F


Product description

The SARS-CoV-2 Triplex PCR kit is one-step reverse transcription real-time PCR multiplex test for detection of SARS-CoV-2 nucleic acids from nasopharyngeal swabs, oropharyngeal swabs and saliva samples from individuals suspected of COVID-19.

SARS-CoV-2 Triplex PCR kit simultaneously detects three targets, in ORF1ab, ORF8, and N protein coding regions, each on separate detection channel. The detection of 3 regions increases the specificity of the test and makes it stable against potential mutations of the virus in the regions where primers or probes bind.

As internal control of quality of RNA extraction and reverse transcription reaction, the test uses specific set of primers and fluorescent probes for transcript of one of the human “housekeeping genes”.

Positive control reaction uses mix of in vitro transcribed RNAs corresponding to the target SARS-CoV-2 genome regions.

Flyer: SARS-CoV-2 Triplex PCR kit

Protocol: SARS-CoV-2-Triplex-PCR-PRTsars-cov-2_triplex_pcr-meridian-healthcare